Isolation and Culture of Mouse Kupffer Cells
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چکیده
منابع مشابه
Mass isolation and culture of rat kupffer cells
Collagenase perfusion of the liver followed by pronase treatment of the cell suspension thus obtained gave a quantitative recovery of viable nonparenchymal liver cells (NPC). From these NPC, Kupffer (K) cells can be purified by attachment to tissue culture dishes. Tail vein injection of carbon 1-2 h before liver perfusion permitted stepwise calculation as well as visualization of carbon-contain...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2023
ISSN: ['1940-087X']
DOI: https://doi.org/10.3791/21515